Read 18240141 mapped reads for 642294 distinct barcodes (Skipped 10617 reads with qBeg > 3) 289318 barcodes seen 10 or more times, map 278438 (minFrac 0.75 minRatio 8) FewGood 8909 0.0308 LoRatio 643 0.0022 NoCons 1198 0.0041 PastEnd 130 0.0004 Usable 278438 0.9624 Masked 183 off-by-1 barcodes (3741 reads) leaving 278255 barcodes Reads for those barcodes: 16220332 of 18240141 (88.9%) Chao2 estimate of #barcodes present (may be inflated for sequencing error): 1322515 278255 insertions in genome are at 166987 different locations Found 186423 insertions (110368 distinct locations) in central 10-90% of genes Found central insertions for 4090 of 4625 protein-coding genes Hit rate in (crude) likely essentials: 0.20 other 0.89 Wrote proteins of 300nt or more with no good insertions to g/Cola/Cola_ML5_comb.unhit Wrote 18 genes with surprising insertions in central 10-90% to g/Cola/Cola_ML5_comb.surprise Wrote read and strain counts for hit genes to g/Cola/Cola_ML5_comb.hit Strains per hit protein: median 32 mean 45.6 Gene and transposon on same strand: 48.2% Reads per hit protein: median 1836 mean 2733.7 bias (ratio) 1.49 Reads per million for hit proteins: median 100.66 mean 149.87