Read 23607068 mapped reads for 642577 distinct barcodes (Skipped 32098 reads with qBeg > 3) 170499 barcodes seen 10 or more times, map 167348 (minFrac 0.75 minRatio 8) FewGood 1838 0.0108 LoRatio 336 0.0020 NoCons 938 0.0055 PastEnd 39 0.0002 Usable 167348 0.9815 Masked 367 off-by-1 barcodes (8464 reads) leaving 166981 barcodes Reads for those barcodes: 22500261 of 23607068 (95.3%) Chao2 estimate of #barcodes present (may be inflated for sequencing error): 3831527 166981 insertions in genome are at 45845 different locations Found 111930 insertions (29620 distinct locations) in central 10-90% of genes Found central insertions for 2584 of 3210 protein-coding genes Hit rate in (crude) likely essentials: 0.15 other 0.81 Wrote proteins of 300nt or more with no good insertions to g/Brev2/Brev2_ML6_comb.pool.unhit Wrote 14 genes with surprising insertions in central 10-90% to g/Brev2/Brev2_ML6_comb.pool.surprise Wrote read and strain counts for hit genes to g/Brev2/Brev2_ML6_comb.pool.hit Strains per hit protein: median 27 mean 43.3 Gene and transposon on same strand: 49.9% Reads per hit protein: median 3460 mean 5820.3 bias (ratio) 1.68 Reads per million for hit proteins: median 146.57 mean 246.55