SetName Date_pool_expt_started Drop Person Mutant Library Description gDNA plate gDNA well Index Sequenced At Media Growth Method Group Temperature pH Liquid v. solid Aerobic_v_Anaerobic Shaking Condition_1 Concentration_1 Units_1 Condition_2 Concentration_2 Units_2 Timecourse Timecourse Sample Growth Plate ID Growth Plate wells StartOD EndOD Total Generations MR1_ML3_set1 8/18/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate10 A1 H1 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.72 6.426264755 MR1_ML3_set1 8/18/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate10 A2 H2 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.72 6.426264755 MR1_ML3_set1 8/18/2013 NA Adam MR1_ML3 LB FEBA_gDNA_plate10 B3 H15 EBB LB 48 well microplate; Tecan Infinite F200 LB 30 NA Liquid Aerobic orbital NA NA NA NA 647 F7,F8 NA NA NA MR1_ML3_set2 8/18/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate10 C1 H25 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.72 6.426264755 MR1_ML3_set2 8/18/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate10 C2 H26 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.72 6.426264755 MR1_ML3_set2 8/18/2013 NA Adam MR1_ML3 LB FEBA_gDNA_plate10 C5 H29 EBB LB 48 well microplate; Tecan Infinite F200 LB 30 NA Liquid Aerobic orbital NA NA NA NA 648 F7,F8 NA NA NA MR1_ML3_set2 8/18/2013 NA Adam MR1_ML3 LB FEBA_gDNA_plate10 D8 H44 EBB LB 48 well microplate; Tecan Infinite F200 LB 30 NA Liquid Aerobic orbital NA NA NA NA 649 F7,F8 NA NA NA MR1_ML3_set2 8/18/2013 NA Adam MR1_ML3 Sodium L-Lactate carbon source replicate 1 FEBA_gDNA_plate10 D9 H45 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Sodium L-Lactate 20 mM NA NA NA NA 0.02 0.662 5.048759312 MR1_ML3_set2 8/18/2013 NA Adam MR1_ML3 Sodium pyruvate carbon source replicate 1 FEBA_gDNA_plate10 D10 H46 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Sodium pyruvate 20 mM NA NA NA NA 0.02 0.26 3.700439718 MR1_ML3_set2 8/18/2013 NA Adam MR1_ML3 Sodium propionate carbon source replicate 1 FEBA_gDNA_plate10 D11 H47 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Sodium propionate 10 mM NA NA NA NA 0.02 0.238 3.572889668 MR1_ML3_set2 8/18/2013 NA Adam MR1_ML3 Tween 20 carbon source replicate 1 FEBA_gDNA_plate10 D12 H48 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Tween 20 0.5 vol% NA NA NA NA 0.02 0.386 4.270528942 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate10 E1 H1 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.72 6.426264755 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 L-Malic acid disodium salt monohydrate carbon source replicate 1 FEBA_gDNA_plate10 E2 H2 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm L-Malic acid disodium salt monohydrate 10 mM NA NA NA NA 0.02 0.356 4.153805336 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 L-Glutamic acid monopotassium salt monohydrate carbon source replicate 1 FEBA_gDNA_plate10 E3 H3 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm L-Glutamic acid monopotassium salt monohydrate 10 mM NA NA NA NA 0.02 1.056 5.722466024 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 L-Glutamine carbon source replicate 1 FEBA_gDNA_plate10 E4 H4 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm L-Glutamine 10 mM NA NA NA NA 0.02 0.346 4.112700133 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 L-Isoleucine carbon source replicate 1 FEBA_gDNA_plate10 E5 H5 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm L-Isoleucine 10 mM NA NA NA NA 0.02 0.742 5.213347282 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 Gly-DL-Asp carbon source replicate 1 FEBA_gDNA_plate10 E6 H6 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Gly-DL-Asp 10 mM NA NA NA NA 0.02 0.3 3.906890596 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 Gly-Glu carbon source replicate 1 FEBA_gDNA_plate10 E7 H7 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Gly-Glu 10 mM NA NA NA NA 0.02 0.304 3.925999419 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 Gelatin carbon source replicate 1 FEBA_gDNA_plate10 E8 H8 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Gelatin 1 mg/mL NA NA NA NA 0.02 0.406 4.343407822 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 casamino acids carbon source replicate 1 FEBA_gDNA_plate10 E9 H9 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm casamino acids 1 mg/mL NA NA NA NA 0.02 0.262 3.711494907 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 N-Acetyl-D-Glucosamine carbon source replicate 1 FEBA_gDNA_plate10 E10 H10 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm N-Acetyl-D-Glucosamine 20 mM NA NA NA NA 0.02 0.622 4.958842675 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 Adenosine carbon source replicate 1 FEBA_gDNA_plate10 E11 H11 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Adenosine 5 mM NA NA NA NA 0.02 0.666 5.057450272 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 Uridine carbon source replicate 1 FEBA_gDNA_plate10 E12 H12 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Uridine 10 mM NA NA NA NA 0.02 0.404 4.336283388 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 Inosine carbon source replicate 1 FEBA_gDNA_plate10 F1 H13 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Inosine 7.5 mM NA NA NA NA 0.02 0.884 5.465974465 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 Cytidine carbon source replicate 1 FEBA_gDNA_plate10 F2 H14 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Cytidine 10 mM NA NA NA NA 0.02 0.418 4.385431037 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 LB FEBA_gDNA_plate10 F3 H15 EBB LB tube LB 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 3.68 7.523561956 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 Sodium L-Lactate carbon source replicate 2 FEBA_gDNA_plate10 F4 H16 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Sodium L-Lactate 20 mM NA NA NA NA 0.02 0.896 5.485426827 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 Sodium D,L-Lactate carbon source replicate 2 FEBA_gDNA_plate10 F5 H17 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Sodium D,L-Lactate 20 mM NA NA NA NA 0.02 0.376 4.232660757 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 Sodium pyruvate carbon source replicate 2 FEBA_gDNA_plate10 F6 H18 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Sodium pyruvate 20 mM NA NA NA NA 0.02 0.252 3.655351829 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 Sodium propionate carbon source replicate 2 FEBA_gDNA_plate10 F7 H19 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Sodium propionate 10 mM NA NA NA NA 0.02 0.242 3.596935142 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 Tween 20 carbon source replicate 2 FEBA_gDNA_plate10 F8 H20 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Tween 20 0.5 vol% NA NA NA NA 0.02 0.322 4.008988783 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 L-Glutamic acid monopotassium salt monohydrate carbon source replicate 2 FEBA_gDNA_plate10 F9 H21 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm L-Glutamic acid monopotassium salt monohydrate 10 mM NA NA NA NA 0.02 0.908 5.504620392 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 L-Glutamine carbon source replicate 2 FEBA_gDNA_plate10 F10 H22 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm L-Glutamine 10 mM NA NA NA NA 0.02 0.438 4.452858965 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 L-Isoleucine carbon source replicate 2 FEBA_gDNA_plate10 F11 H23 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm L-Isoleucine 10 mM NA NA NA NA 0.02 0.896 5.485426827 MR1_ML3_set3 8/18/2013 NA Adam MR1_ML3 Gly-DL-Asp carbon source replicate 2 FEBA_gDNA_plate10 F12 H24 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Gly-DL-Asp 10 mM NA NA NA NA 0.02 0.334 4.061776198 MR1_ML3_set4 8/18/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate10 G1 H25 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.72 6.426264755 MR1_ML3_set4 8/18/2013 NA Adam MR1_ML3 Gly-Glu carbon source replicate 2 FEBA_gDNA_plate10 G2 H26 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Gly-Glu 10 mM NA NA NA NA 0.02 0.326 4.026800059 MR1_ML3_set4 8/18/2013 NA Adam MR1_ML3 Gelatin carbon source replicate 2 FEBA_gDNA_plate10 G3 H27 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Gelatin 1 mg/mL NA NA NA NA 0.02 0.262 3.711494907 MR1_ML3_set4 8/18/2013 NA Adam MR1_ML3 casamino acids carbon source replicate 2 FEBA_gDNA_plate10 G4 H28 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm casamino acids 1 mg/mL NA NA NA NA 0.02 0.264 3.722466024 MR1_ML3_set4 8/18/2013 NA Adam MR1_ML3 N-Acetyl-D-Glucosamine carbon source replicate 2 FEBA_gDNA_plate10 G5 H29 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm N-Acetyl-D-Glucosamine 20 mM NA NA NA NA 0.02 0.992 5.632268215 MR1_ML3_set4 8/18/2013 NA Adam MR1_ML3 Adenosine carbon source replicate 2 FEBA_gDNA_plate10 G6 H30 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Adenosine 5 mM NA NA NA NA 0.02 0.676 5.078951341 MR1_ML3_set4 8/18/2013 NA Adam MR1_ML3 Uridine carbon source replicate 2 FEBA_gDNA_plate10 G7 H31 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Uridine 10 mM NA NA NA NA 0.02 0.436 4.44625623 MR1_ML3_set4 8/18/2013 NA Adam MR1_ML3 Inosine carbon source replicate 2 FEBA_gDNA_plate10 G8 H32 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Inosine 7.5 mM NA NA NA NA 0.02 0.888 5.472487771 MR1_ML3_set4 8/18/2013 NA Adam MR1_ML3 Cytidine carbon source replicate 2 FEBA_gDNA_plate10 G9 H33 EBB ShewMM_noCarbon tube carbon source 30 7 Liquid Aerobic 200 rpm Cytidine 10 mM NA NA NA NA 0.02 0.398 4.314696526 MR1_ML3_set4 8/18/2013 NA Adam MR1_ML3 LB FEBA_gDNA_plate10 G10 H34 EBB LB tube LB 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 3.65 7.511752654 MR1_ML3_set4 8/19/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate10 G11 H35 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.246 5.961160258 MR1_ML3_set4 8/19/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate10 G12 H36 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.246 5.961160258 MR1_ML3_set4 8/19/2013 NA Adam MR1_ML3 LB FEBA_gDNA_plate10 H7 H43 EBB LB 48 well microplate; Tecan Infinite F200 LB 30 NA Liquid Aerobic orbital NA NA NA NA 651 F7,F8 NA NA NA MR1_ML3_set4 8/19/2013 NA Adam MR1_ML3 LB FEBA_gDNA_plate10 H12 H48 EBB LB 48 well microplate; Tecan Infinite F200 LB 30 NA Liquid Aerobic orbital NA NA NA NA 650 F5,F6 NA NA NA MR1_ML3_set5 8/19/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate14 E1 H1 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.246 5.961160258 MR1_ML3_set5 8/19/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate14 E2 H2 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.246 5.961160258 MR1_ML3_set5 8/19/2013 NA Adam MR1_ML3 LB FEBA_gDNA_plate14 E12 H12 EBB LB 48 well microplate; Tecan Infinite F200 LB 30 NA Liquid Aerobic orbital NA NA NA NA 652 F7,F8 NA NA NA MR1_ML3_set5 8/21/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate14 F1 H13 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.364 6.091699834 MR1_ML3_set5 8/21/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate14 F2 H14 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.364 6.091699834 MR1_ML3_set5 8/21/2013 NA Adam MR1_ML3 LB FEBA_gDNA_plate14 H8 H44 EBB LB 48 well microplate; Tecan Infinite F200 LB 30 NA Liquid Aerobic orbital NA NA NA NA 653 F7,F8 NA NA NA MR1_ML3_set5 8/21/2013 NA Adam MR1_ML3 LB FEBA_gDNA_plate14 H11 H47 EBB LB 48 well microplate; Tecan Infinite F200 LB 30 NA Liquid Aerobic orbital NA NA NA NA 654 F7,F8 NA NA NA MR1_ML3_set5 8/21/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate14 H12 H48 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.364 6.091699834 MR1_ML3_set6 10/22/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate16 A1 H1 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.22 5.930737338 MR1_ML3_set6 10/22/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate16 A2 H2 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.22 5.930737338 MR1_ML3_set6 10/22/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate16 A3 H3 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.22 5.930737338 MR1_ML3_set6 10/22/2013 NA Adam MR1_ML3 LB FEBA_gDNA_plate16 A7 H7 EBB LB 48 well microplate; Tecan Infinite F200 LB 30 NA Liquid Aerobic orbital NA NA NA NA 754 C7,C8 NA NA NA MR1_ML3_set6 10/22/2013 NA Adam MR1_ML3 LB FEBA_gDNA_plate16 A11 H11 EBB LB 48 well microplate; Tecan Infinite F200 LB 30 NA Liquid Aerobic orbital NA NA NA NA 754 F7,F8 NA NA NA MR1_ML3_set6 10/22/2013 NA Adam MR1_ML3 LB FEBA_gDNA_plate16 B6 H18 EBB LB 48 well microplate; Tecan Infinite F200 LB 30 NA Liquid Aerobic orbital NA NA NA NA 755 C7,C8 NA NA NA MR1_ML3_set6 10/22/2013 NA Adam MR1_ML3 LB FEBA_gDNA_plate16 C1 H25 EBB LB 48 well microplate; Tecan Infinite F200 LB 30 NA Liquid Aerobic orbital NA NA NA NA 755 F7,F8 NA NA NA MR1_ML3_set6 10/22/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate16 D10 H46 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.22 5.930737338 MR1_ML3_set6 10/22/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate16 D11 H47 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.22 5.930737338 MR1_ML3_set6 10/22/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate16 D12 H48 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.22 5.930737338 MR1_SB2B_combined_set 8/28/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate16 E1 H1 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.56 6.285402219 MR1_SB2B_combined_set 8/28/2013 NA Adam MR1_ML3 Time0 FEBA_gDNA_plate16 E2 H2 EBB LB flask Time0 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 1.56 6.285402219 MR1_SB2B_combined_set 8/28/2013 NA Adam MR1_ML3 LB 30C FEBA_gDNA_plate16 F8 H20 EBB LB tube temperature 30 NA Liquid Aerobic 200 rpm NA NA NA NA NA 0.02 NA NA