SetName Date_pool_expt_started Drop Person Mutant.Library Description gDNA.plate gDNA.well Index Sequenced.At Media Growth.Method Group Temperature pH Liquid.v..solid Aerobic_v_Anaerobic Shaking Condition_1 Concentration_1 Units_1 Condition_2 Concentration_2 Units_2 Timecourse Timecourse.Sample Growth.Plate.ID Growth.Plate.wells StartOD EndOD Total.Generations num name short SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Time0 FEBA_gDNA_plate1 A1 H1 JGI LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.1 1.74 4.12 1 set1H1 Time0 SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Time0 FEBA_gDNA_plate1 A2 H2 JGI LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.1 1.74 4.12 2 set1H2 Time0 SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with D-Glucose as carbon source FEBA_gDNA_plate1 A3 H3 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital D-Glucose 20 mM NA NA NA NA 509 A1,A2 NA NA NA 3 set1H3 D-Glucose SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with D-Maltose monohydrate as carbon source FEBA_gDNA_plate1 A4 H4 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital D-Maltose monohydrate 20 mM NA NA NA NA 509 A3,A4 NA NA NA 4 set1H4 D-Maltose SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with a-Ketoglutaric acid disodium salt hydrate as carbon source FEBA_gDNA_plate1 A5 H5 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital a-Ketoglutaric acid disodium salt hydrate 20 mM NA NA NA NA 509 A5,A6 NA NA NA 5 set1H5 a-Ketoglutaric SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Potassium acetate as carbon source FEBA_gDNA_plate1 A6 H6 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Potassium acetate 20 mM NA NA NA NA 509 A7,A8 NA NA NA 6 set1H6 acetate SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Sodium Fumarate dibasic as carbon source FEBA_gDNA_plate1 A7 H7 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium Fumarate dibasic 20 mM NA NA NA NA 509 B1,B2 NA NA NA 7 set1H7 Fumarate SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with D-Cellobiose as carbon source FEBA_gDNA_plate1 A8 H8 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital D-Cellobiose 20 mM NA NA NA NA 509 B3,B4 NA NA NA 8 set1H8 D-Cellobiose SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Sodium L-Lactate as carbon source FEBA_gDNA_plate1 A9 H9 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium L-Lactate 20 mM NA NA NA NA 509 B5,B6 NA NA NA 9 set1H9 L-Lactate SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Sodium D,L-Lactate as carbon source FEBA_gDNA_plate1 A10 H10 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium D,L-Lactate 20 mM NA NA NA NA 509 B7,B8 NA NA NA 10 set1H10 D,L-Lactate SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Sodium pyruvate as carbon source FEBA_gDNA_plate1 A11 H11 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium pyruvate 20 mM NA NA NA NA 509 C1,C2 NA NA NA 11 set1H11 pyruvate SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Sodium succinate dibasic hexahydrate as carbon source FEBA_gDNA_plate1 A12 H12 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium succinate dibasic hexahydrate 20 mM NA NA NA NA 509 C3,C4 NA NA NA 12 set1H12 succinate SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with D-Mannitol as carbon source FEBA_gDNA_plate1 B1 H13 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital D-Mannitol 20 mM NA NA NA NA 509 C7,C8 NA NA NA 13 set1H13 D-Mannitol SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Tween 20 as carbon source FEBA_gDNA_plate1 B2 H14 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Tween 20 1 % NA NA NA NA 509 D1,D2 NA NA NA 14 set1H14 Tween 20 SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with L-Malic acid disodium salt monohydrate as carbon source FEBA_gDNA_plate1 B3 H15 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital L-Malic acid disodium salt monohydrate 20 mM NA NA NA NA 509 D3,D4 NA NA NA 15 set1H15 L-Malic SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with L-Glutamic acid monopotassium salt monohydrate as carbon source FEBA_gDNA_plate1 B4 H16 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital L-Glutamic acid monopotassium salt monohydrate 20 mM NA NA NA NA 509 D7,D8 NA NA NA 16 set1H16 L-Glutamic SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with L-Glutamine as carbon source FEBA_gDNA_plate1 B5 H17 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital L-Glutamine 20 mM NA NA NA NA 509 E1,E2 NA NA NA 17 set1H17 L-Glutamine SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Gly-Glu as carbon source FEBA_gDNA_plate1 B6 H18 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Gly-Glu 20 mM NA NA NA NA 509 F3,F4 NA NA NA 18 set1H18 Gly-Glu SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Gelatin as carbon source FEBA_gDNA_plate1 B7 H19 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Gelatin 1 mg/ml NA NA NA NA 509 F5,F6 NA NA NA 19 set1H19 Gelatin SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with CAS amino acids as carbon source FEBA_gDNA_plate1 B8 H20 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital CAS amino acids 5 mg/ml NA NA NA NA 507 A1,A2 NA NA NA 20 set1H20 CAS aminos SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Putrescine Dihydrochloride as carbon source FEBA_gDNA_plate1 B9 H21 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Putrescine Dihydrochloride 20 mM NA NA NA NA 507 A3,A4 NA NA NA 21 set1H21 Putrescine SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with N-Acetyl-D-Glucosamine as carbon source FEBA_gDNA_plate1 B10 H22 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital N-Acetyl-D-Glucosamine 20 mM NA NA NA NA 507 A5,A6 NA NA NA 22 set1H22 NAG SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Adenosine as carbon source FEBA_gDNA_plate1 B11 H23 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Adenosine 5 mM NA NA NA NA 507 A7,A8 NA NA NA 23 set1H23 Adenosine SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Uridine as carbon source FEBA_gDNA_plate1 B12 H24 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Uridine 20 mM NA NA NA NA 507 B1,B2 NA NA NA 24 set1H24 Uridine SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Thymidine as carbon source FEBA_gDNA_plate1 C1 H25 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Thymidine 10 mM NA NA NA NA 507 B3,B4 NA NA NA 25 set1H25 Thymidine SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Inosine as carbon source FEBA_gDNA_plate1 C2 H26 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Inosine 12.5 mM NA NA NA NA 507 B5,B6 NA NA NA 26 set1H26 Inosine SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Cytidine as carbon source FEBA_gDNA_plate1 C3 H27 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Cytidine 20 mM NA NA NA NA 507 B7,B8 NA NA NA 27 set1H27 Cytidine SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with D-Mannose as carbon source FEBA_gDNA_plate1 C4 H28 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital D-Mannose 20 mM NA NA NA NA 507 C1,C2 NA NA NA 28 set1H28 D-Mannose SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Sucrose as carbon source FEBA_gDNA_plate1 C5 H29 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sucrose 20 mM NA NA NA NA 507 C5,C6 NA NA NA 29 set1H29 Sucrose SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with L-Serine as carbon source FEBA_gDNA_plate1 C6 H30 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital L-Serine 20 mM NA NA NA NA 507 C7,C8 NA NA NA 30 set1H30 L-Serine SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with D-Glucose as carbon source FEBA_gDNA_plate1 C7 H31 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital D-Glucose 20 mM NA NA NA NA 509 A1,A2 NA NA NA 31 set1H31 D-Glucose SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Sodium D,L-Lactate as carbon source FEBA_gDNA_plate1 C8 H32 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium D,L-Lactate 20 mM NA NA NA NA 509 B7,B8 NA NA NA 32 set1H32 D,L-Lactate SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Tween 20 as carbon source FEBA_gDNA_plate1 C9 H33 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Tween 20 1 % NA NA NA NA 509 D1,D2 NA NA NA 33 set1H33 Tween 20 SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with CAS amino acids as carbon source FEBA_gDNA_plate1 C10 H34 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital CAS amino acids 5 mg/ml NA NA NA NA 507 A1,A2 NA NA NA 34 set1H34 CAS aminos SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with Putrescine Dihydrochloride as carbon source FEBA_gDNA_plate1 C11 H35 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Putrescine Dihydrochloride 20 mM NA NA NA NA 507 A3,A4 NA NA NA 35 set1H35 Putrescine SB2B_ML5_set1 4/15/2013 NA Jake SB2B_ML5 Minimal media with N-Acetyl-D-Glucosamine as carbon source FEBA_gDNA_plate1 C12 H36 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital N-Acetyl-D-Glucosamine 20 mM NA NA NA NA 507 A5,A6 NA NA NA 36 set1H36 NAG SB2B_ML5_set1 3/25/2013 NA Kelly SB2B_ML5 Time0 FEBA_gDNA_plate1 D1 H37 JGI LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.1 1.74 4.12 37 set1H37 Time0 SB2B_ML5_set1 3/25/2013 NA Kelly SB2B_ML5 LB flask, timecourse 1, timepoint 1 FEBA_gDNA_plate1 D2 H38 JGI LB flask LB 37 NA Liquid Aerobic 200 rpm NA NA NA NA LB flask timecourse 1 1 NA 0.02 2.23 6.8 38 set1H38 LB rep1 t1 SB2B_ML5_set1 3/25/2013 NA Kelly SB2B_ML5 LB flask, timecourse 1, timepoint 2 FEBA_gDNA_plate1 D3 H39 JGI LB flask LB 37 NA Liquid Aerobic 200 rpm NA NA NA NA LB flask timecourse 1 2 NA 0.02 1.84 13.32 39 set1H39 LB rep1 t2 SB2B_ML5_set1 3/25/2013 NA Kelly SB2B_ML5 LB flask, timecourse 2, timepoint 1 FEBA_gDNA_plate1 D4 H40 JGI LB flask LB 37 NA Liquid Aerobic 200 rpm NA NA NA NA LB flask timecourse 2 1 NA 0.02 2.17 6.76 40 set1H40 LB rep2 t1 SB2B_ML5_set1 3/25/2013 NA Kelly SB2B_ML5 LB flask, timecourse 2, timepoint 2 FEBA_gDNA_plate1 D5 H41 JGI LB flask LB 37 NA Liquid Aerobic 200 rpm NA NA NA NA LB flask timecourse 2 2 NA 0.02 1.85 13.29 41 set1H41 LB rep2 t2 SB2B_ML5_set1 3/25/2013 NA Kelly SB2B_ML5 LB, microplate FEBA_gDNA_plate1 D6 H42 JGI LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 506 C4 NA NA NA 42 set1H42 LB, microplate SB2B_ML5_set1 3/25/2013 NA Kelly SB2B_ML5 LB, microplate FEBA_gDNA_plate1 D7 H43 JGI LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 506 C1,C2 NA NA NA 43 set1H43 LB, microplate SB2B_ML5_set1 3/25/2013 NA Kelly SB2B_ML5 LB, microplate FEBA_gDNA_plate1 D8 H44 JGI LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 506 B1,B2,B3,B4 NA NA NA 44 set1H44 LB, microplate SB2B_ML5_set1 3/25/2013 NA Kelly SB2B_ML5 Minimal media with Sodium D,L-Lactate as carbon source FEBA_gDNA_plate1 D9 H45 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium D,L-Lactate 20 mM NA NA NA NA 506 F5 NA NA NA 45 set1H45 D,L-Lactate SB2B_ML5_set1 3/25/2013 NA Kelly SB2B_ML5 Minimal media with Sodium D,L-Lactate as carbon source FEBA_gDNA_plate1 D10 H46 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium D,L-Lactate 20 mM NA NA NA NA 506 F6,F7 NA NA NA 46 set1H46 D,L-Lactate SB2B_ML5_set1 3/25/2013 NA Kelly SB2B_ML5 Minimal media with Sodium D,L-Lactate as carbon source FEBA_gDNA_plate1 D11 H47 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium D,L-Lactate 20 mM NA NA NA NA 506 D1,D2,D3,D4 NA NA NA 47 set1H47 D,L-Lactate SB2B_ML5_set1 3/25/2013 NA Kelly SB2B_ML5 Minimal media with Sodium D,L-Lactate as carbon source FEBA_gDNA_plate1 D12 H48 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium D,L-Lactate 20 mM NA NA NA NA 506 D5,D6,D7,D8 NA NA NA 48 set1H48 D,L-Lactate SB2B_ML5_set2 3/25/2013 NA Kelly SB2B_ML5 Time0 FEBA_gDNA_plate1 E1 H1 JGI LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.1 1.74 4.12 49 set2H1 Time0 SB2B_ML5_set2 3/25/2013 NA Kelly SB2B_ML5 LB flask, timecourse 1, timepoint 1 FEBA_gDNA_plate1 E2 H2 JGI LB flask LB 37 NA Liquid Aerobic 200 rpm NA NA NA NA LB flask timecourse 1 1 NA 0.02 2.23 6.8 50 set2H2 LB rep1 t1 SB2B_ML5_set2 3/25/2013 NA Kelly SB2B_ML5 LB flask, timecourse 1, timepoint 2 FEBA_gDNA_plate1 E3 H3 JGI LB flask LB 37 NA Liquid Aerobic 200 rpm NA NA NA NA LB flask timecourse 1 2 NA 0.02 1.84 13.32 51 set2H3 LB rep1 t2 SB2B_ML5_set2 3/25/2013 NA Kelly SB2B_ML5 LB flask, timecourse 2, timepoint 1 FEBA_gDNA_plate1 E4 H4 JGI LB flask LB 37 NA Liquid Aerobic 200 rpm NA NA NA NA LB flask timecourse 2 1 NA 0.02 2.17 6.76 52 set2H4 LB rep2 t1 SB2B_ML5_set2 3/25/2013 NA Kelly SB2B_ML5 LB flask, timecourse 2, timepoint 2 FEBA_gDNA_plate1 E5 H5 JGI LB flask LB 37 NA Liquid Aerobic 200 rpm NA NA NA NA LB flask timecourse 2 2 NA 0.02 1.85 13.29 53 set2H5 LB rep2 t2 SB2B_ML5_set2 3/25/2013 NA Kelly SB2B_ML5 LB, microplate FEBA_gDNA_plate1 E6 H6 JGI LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 506 C5 NA NA NA 54 set2H6 LB, microplate SB2B_ML5_set2 3/25/2013 NA Kelly SB2B_ML5 LB, microplate FEBA_gDNA_plate1 E7 H7 JGI LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 506 C7,C8 NA NA NA 55 set2H7 LB, microplate SB2B_ML5_set2 3/25/2013 NA Kelly SB2B_ML5 LB, microplate FEBA_gDNA_plate1 E8 H8 JGI LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 506 B5,B6,B7,B8 NA NA NA 56 set2H8 LB, microplate SB2B_ML5_set2 3/25/2013 NA Kelly SB2B_ML5 Minimal media with Sodium D,L-Lactate as carbon source FEBA_gDNA_plate1 E9 H9 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium D,L-Lactate 20 mM NA NA NA NA 506 F8 NA NA NA 57 set2H9 D,L-Lactate SB2B_ML5_set2 3/25/2013 NA Kelly SB2B_ML5 Minimal media with Sodium D,L-Lactate as carbon source FEBA_gDNA_plate1 E10 H10 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium D,L-Lactate 20 mM NA NA NA NA 506 E7,E8,F1,F2,F3,F4 NA NA NA 58 set2H10 D,L-Lactate SB2B_ML5_set2 3/25/2013 NA Kelly SB2B_ML5 LB, microplate FEBA_gDNA_plate1 E11 H11 JGI LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 506 C6 NA NA NA 59 set2H11 LB, microplate SB2B_ML5_set2 3/25/2013 NA Kelly SB2B_ML5 LB, microplate FEBA_gDNA_plate1 E12 H12 JGI LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 506 A1,A2,A3,A4,A5,A6 NA NA NA 60 set2H12 LB, microplate SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Time0 FEBA_gDNA_plate1 F1 H13 JGI LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.1 1.74 4.12 61 set2H13 Time0 SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Time0 FEBA_gDNA_plate1 F2 H14 JGI LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.1 1.74 4.12 62 set2H14 Time0 SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with D-Glucose as carbon source FEBA_gDNA_plate1 F3 H15 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital D-Glucose 20 mM NA NA NA NA 505 A1,A2 NA NA NA 63 set2H15 D-Glucose SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with D-Maltose monohydrate as carbon source FEBA_gDNA_plate1 F4 H16 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital D-Maltose monohydrate 20 mM NA NA NA NA 505 A3,A4 NA NA NA 64 set2H16 D-Maltose SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with a-Ketoglutaric acid disodium salt hydrate as carbon source FEBA_gDNA_plate1 F5 H17 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital a-Ketoglutaric acid disodium salt hydrate 20 mM NA NA NA NA 505 A5,A6 NA NA NA 65 set2H17 a-Ketoglutaric SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Potassium acetate as carbon source FEBA_gDNA_plate1 F6 H18 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Potassium acetate 20 mM NA NA NA NA 505 A7,A8 NA NA NA 66 set2H18 acetate SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Sodium Fumarate dibasic as carbon source FEBA_gDNA_plate1 F7 H19 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium Fumarate dibasic 20 mM NA NA NA NA 505 B1,B2 NA NA NA 67 set2H19 Fumarate SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with D-Cellobiose as carbon source FEBA_gDNA_plate1 F8 H20 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital D-Cellobiose 20 mM NA NA NA NA 505 B3,B4 NA NA NA 68 set2H20 D-Cellobiose SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Sodium L-Lactate as carbon source FEBA_gDNA_plate1 F9 H21 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium L-Lactate 20 mM NA NA NA NA 505 B5,B6 NA NA NA 69 set2H21 L-Lactate SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Sodium D,L-Lactate as carbon source FEBA_gDNA_plate1 F10 H22 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium D,L-Lactate 20 mM NA NA NA NA 505 B7,B8 NA NA NA 70 set2H22 D,L-Lactate SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Sodium pyruvate as carbon source FEBA_gDNA_plate1 F11 H23 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium pyruvate 20 mM NA NA NA NA 505 C1,C2 NA NA NA 71 set2H23 pyruvate SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Sodium succinate dibasic hexahydrate as carbon source FEBA_gDNA_plate1 F12 H24 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium succinate dibasic hexahydrate 20 mM NA NA NA NA 505 C3,C4 NA NA NA 72 set2H24 succinate SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with D-Mannitol as carbon source FEBA_gDNA_plate1 G1 H25 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital D-Mannitol 20 mM NA NA NA NA 505 C7,C8 NA NA NA 73 set2H25 D-Mannitol SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Tween 20 as carbon source FEBA_gDNA_plate1 G2 H26 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Tween 20 1 % NA NA NA NA 505 D1,D2 NA NA NA 74 set2H26 Tween 20 SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with L-Malic acid disodium salt monohydrate as carbon source FEBA_gDNA_plate1 G3 H27 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital L-Malic acid disodium salt monohydrate 20 mM NA NA NA NA 505 D3,D4 NA NA NA 75 set2H27 L-Malic SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with L-Glutamic acid monopotassium salt monohydrate as carbon source FEBA_gDNA_plate1 G4 H28 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital L-Glutamic acid monopotassium salt monohydrate 20 mM NA NA NA NA 505 D7,D8 NA NA NA 76 set2H28 L-Glutamic SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with L-Glutamine as carbon source FEBA_gDNA_plate1 G5 H29 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital L-Glutamine 20 mM NA NA NA NA 505 E1,E2 NA NA NA 77 set2H29 L-Glutamine SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Gly-Glu as carbon source FEBA_gDNA_plate1 G6 H30 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Gly-Glu 20 mM NA NA NA NA 505 F3,F4 NA NA NA 78 set2H30 Gly-Glu SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Gelatin as carbon source FEBA_gDNA_plate1 G7 H31 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Gelatin 1 mg/ml NA NA NA NA 505 F5,F6 NA NA NA 79 set2H31 Gelatin SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with CAS amino acids as carbon source FEBA_gDNA_plate1 G8 H32 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital CAS amino acids 5 mg/ml NA NA NA NA 508 A1,A2 NA NA NA 80 set2H32 CAS aminos SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Putrescine Dihydrochloride as carbon source FEBA_gDNA_plate1 G9 H33 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Putrescine Dihydrochloride 20 mM NA NA NA NA 508 A3,A4 NA NA NA 81 set2H33 Putrescine SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with N-Acetyl-D-Glucosamine as carbon source FEBA_gDNA_plate1 G10 H34 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital N-Acetyl-D-Glucosamine 20 mM NA NA NA NA 508 A5,A6 NA NA NA 82 set2H34 NAG SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Adenosine as carbon source FEBA_gDNA_plate1 G11 H35 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Adenosine 5 mM NA NA NA NA 508 A7,A8 NA NA NA 83 set2H35 Adenosine SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Uridine as carbon source FEBA_gDNA_plate1 G12 H36 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Uridine 20 mM NA NA NA NA 508 B1,B2 NA NA NA 84 set2H36 Uridine SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Thymidine as carbon source FEBA_gDNA_plate1 H1 H37 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Thymidine 10 mM NA NA NA NA 508 B3,B4 NA NA NA 85 set2H37 Thymidine SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Inosine as carbon source FEBA_gDNA_plate1 H2 H38 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Inosine 12.5 mM NA NA NA NA 508 B5,B6 NA NA NA 86 set2H38 Inosine SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Cytidine as carbon source FEBA_gDNA_plate1 H3 H39 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Cytidine 20 mM NA NA NA NA 508 B7,B8 NA NA NA 87 set2H39 Cytidine SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with D-Mannose as carbon source FEBA_gDNA_plate1 H4 H40 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital D-Mannose 20 mM NA NA NA NA 508 C1,C2 NA NA NA 88 set2H40 D-Mannose SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Sucrose as carbon source FEBA_gDNA_plate1 H5 H41 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sucrose 20 mM NA NA NA NA 508 C5,C6 NA NA NA 89 set2H41 Sucrose SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with L-Serine as carbon source FEBA_gDNA_plate1 H6 H42 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital L-Serine 20 mM NA NA NA NA 508 C7,C8 NA NA NA 90 set2H42 L-Serine SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with D-Glucose as carbon source FEBA_gDNA_plate1 H7 H43 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital D-Glucose 20 mM NA NA NA NA 505 A1,A2 NA NA NA 91 set2H43 D-Glucose SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Sodium D,L-Lactate as carbon source FEBA_gDNA_plate1 H8 H44 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Sodium D,L-Lactate 20 mM NA NA NA NA 505 B7,B8 NA NA NA 92 set2H44 D,L-Lactate SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Tween 20 as carbon source FEBA_gDNA_plate1 H9 H45 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Tween 20 1 % NA NA NA NA 505 D1,D2 NA NA NA 93 set2H45 Tween 20 SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with CAS amino acids as carbon source FEBA_gDNA_plate1 H10 H46 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital CAS amino acids 5 mg/ml NA NA NA NA 508 A1,A2 NA NA NA 94 set2H46 CAS aminos SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with Putrescine Dihydrochloride as carbon source FEBA_gDNA_plate1 H11 H47 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital Putrescine Dihydrochloride 20 mM NA NA NA NA 508 A3,A4 NA NA NA 95 set2H47 Putrescine SB2B_ML5_set2 4/15/2013 NA Jake SB2B_ML5 Minimal media with N-Acetyl-D-Glucosamine as carbon source FEBA_gDNA_plate1 H12 H48 JGI ShewMM_noCarbon 48 well microplate; Tecan Infinite F200 carbon source 37 7 Liquid Aerobic orbital N-Acetyl-D-Glucosamine 20 mM NA NA NA NA 508 A5,A6 NA NA NA 96 set2H48 NAG SB2B_ML5_set4 7/26/2013 NA Adam SB2B_ML5 LB FEBA_gDNA_plate6 E10 H10 EBB LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 588 F7,F8 NA NA NA 97 set4H10 LB SB2B_ML5_set4 7/26/2013 NA Adam SB2B_ML5 Time0 FEBA_gDNA_plate6 F7 H19 EBB LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.02 1.71 6.417852515 98 set4H19 Time0 SB2B_ML5_set4 7/26/2013 NA Adam SB2B_ML5 LB FEBA_gDNA_plate6 F8 H20 EBB LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 589 F7,F8 NA NA NA 99 set4H20 LB SB2B_ML5_set4 7/26/2013 NA Adam SB2B_ML5 LB FEBA_gDNA_plate6 G10 H34 EBB LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 590 F7,F8 NA NA NA 100 set4H34 LB SB2B_ML5_set4 7/26/2013 NA Adam SB2B_ML5 LB FEBA_gDNA_plate6 H6 H42 EBB LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 591 F7,F8 NA NA NA 101 set4H42 LB SB2B_ML5_set4 7/26/2013 NA Adam SB2B_ML5 Time0 FEBA_gDNA_plate6 H7 H43 EBB LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.02 1.71 6.417852515 102 set4H43 Time0 SB2B_ML5_set4 7/26/2013 NA Adam SB2B_ML5 Time0 FEBA_gDNA_plate6 H8 H44 EBB LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.02 1.71 6.417852515 103 set4H44 Time0 SB2B_ML5_set5 7/30/2013 NA Adam SB2B_ML5 Time0 FEBA_gDNA_plate14 A1 H1 EBB LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.02 1.72 6.426264755 104 set5H1 Time0 SB2B_ML5_set5 7/30/2013 NA Adam SB2B_ML5 Time0 FEBA_gDNA_plate14 A2 H2 EBB LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.02 1.72 6.426264755 105 set5H2 Time0 SB2B_ML5_set5 7/30/2013 NA Adam SB2B_ML5 LB FEBA_gDNA_plate14 A7 H7 EBB LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 592 F7,F8 NA NA NA 106 set5H7 LB SB2B_ML5_set5 7/30/2013 NA Adam SB2B_ML5 LB FEBA_gDNA_plate14 A11 H11 EBB LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 593 F7,F8 NA NA NA 107 set5H11 LB SB2B_ML5_set5 7/30/2013 NA Adam SB2B_ML5 LB FEBA_gDNA_plate14 B11 H23 EBB LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital 0 mg/ml NA NA NA NA 594 F7,F8 NA NA NA 108 set5H23 LB SB2B_ML5_set5 8/28/2013 NA Kelly SB2B_ML5 Time0 FEBA_gDNA_plate14 B12 H24 EBB LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.02 1.31 6.033423002 109 set5H24 Time0 SB2B_ML5_set5 8/28/2013 NA Kelly SB2B_ML5 Time0 FEBA_gDNA_plate14 C1 H25 EBB LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.02 1.31 6.033423002 110 set5H25 Time0 SB2B_ML5_set5 8/28/2013 NA Adam SB2B_ML5 LB, 37C FEBA_gDNA_plate14 D3 H39 EBB LB tube temperature 37 NA Liquid Aerobic orbital NA NA NA NA NA NA 0.02 4 7.64385619 111 set5H39 LB, 37C MR1_SB2B_combined_set 10/15/2013 NA Adam SB2B_ML5 Time0 FEBA_gDNA_plate16 G6 H30 EBB LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.02 1.43 6.159871337 112 setAH30 Time0 MR1_SB2B_combined_set 10/15/2013 NA Adam SB2B_ML5 Time0 FEBA_gDNA_plate16 G7 H31 EBB LB flask Time0 37 NA Liquid Aerobic 200 rpm NA NA NA NA NA NA 0.02 1.43 6.159871337 113 setAH31 Time0 MR1_SB2B_combined_set 10/15/2013 NA Adam SB2B_ML5 LB FEBA_gDNA_plate16 H5 H41 EBB LB 48 well microplate; Tecan Infinite F200 LB 37 NA Liquid Aerobic orbital NA NA NA NA NA 731 C7,C8 NA NA NA 114 setAH41 LB