SetName Date_pool_expt_started Drop Person Mutant Library Description gDNA plate gDNA well Index Sequenced At Media Growth Method Group Temperature pH Liquid v. solid Aerobic_v_Anaerobic Shaking Condition_1 Concentration_1 Units_1 Condition_2 Concentration_2 Units_2 Timecourse Timecourse Sample Growth Plate ID Growth Plate wells StartOD EndOD Total Generations Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Time0 FEBA_gDNA_plate31 A1 IT001 LB flask Time0 37 Liquid Aerobic 200 rpm 0.02 2.23 6.8008999 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Time0 FEBA_gDNA_plate31 A2 IT002 LB flask Time0 37 Liquid Aerobic 200 rpm 0.02 2.23 6.8008999 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Glucose carbon source FEBA_gDNA_plate31 A3 IT003 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Glucose 20 mM 0.02 1.85 6.529820947 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Glucose carbon source FEBA_gDNA_plate31 A4 IT004 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Glucose 20 mM 0.02 1.84 6.520422249 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Fructose carbon source FEBA_gDNA_plate31 A5 IT005 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Fructose 20 mM 0.02 1.1 5.781359714 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Fructose carbon source FEBA_gDNA_plate31 A6 IT006 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Fructose 20 mM 0.02 1.16 5.857980995 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Sucrose carbon source FEBA_gDNA_plate31 A7 IT007 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Sucrose 20 mM 0.02 1.26 5.977279923 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Sucrose carbon source FEBA_gDNA_plate31 A8 IT008 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Sucrose 20 mM 0.02 1.24 5.95419631 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Maltose monohydrate carbon source FEBA_gDNA_plate31 A9 IT009 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Maltose monohydrate 20 mM 0.02 1.22 5.930737338 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Maltose monohydrate carbon source FEBA_gDNA_plate31 A10 IT010 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Maltose monohydrate 20 mM 0.02 1.25 5.965784285 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Xylose carbon source FEBA_gDNA_plate31 A11 IT011 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Xylose 20 mM 0.02 0.94 5.554588852 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Xylose carbon source FEBA_gDNA_plate31 A12 IT012 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Xylose 20 mM 0.02 0.94 5.554588852 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Galactose carbon source FEBA_gDNA_plate31 B1 IT013 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Galactose 20 mM 0.02 0.7 5.129283017 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Galactose carbon source FEBA_gDNA_plate31 B2 IT014 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Galactose 20 mM 0.02 0.74 5.209453366 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Ribose carbon source FEBA_gDNA_plate31 B3 IT015 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Ribose 20 mM 0.02 1 5.64385619 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Ribose carbon source FEBA_gDNA_plate31 B4 IT016 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Ribose 20 mM 0.02 1.2 5.906890596 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Fucose carbon source FEBA_gDNA_plate31 B5 IT017 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm L-Fucose 20 mM 0.02 0.59 4.882643049 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Fucose carbon source FEBA_gDNA_plate31 B6 IT018 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm L-Fucose 20 mM 0.02 0.61 4.930737338 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 a-Ketoglutaric acid disodium salt hydrate carbon source FEBA_gDNA_plate31 B7 IT019 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm a-Ketoglutaric acid disodium salt hydrate 20 mM 0.02 1.12 5.807354922 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 a-Ketoglutaric acid disodium salt hydrate carbon source FEBA_gDNA_plate31 B8 IT020 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm a-Ketoglutaric acid disodium salt hydrate 20 mM 0.02 1.1 5.781359714 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Glucuronic Acid carbon source FEBA_gDNA_plate31 B9 IT021 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Glucuronic Acid 20 mM 0.02 1.78 6.475733431 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Glucuronic Acid carbon source FEBA_gDNA_plate31 B10 IT022 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Glucuronic Acid 20 mM 0.02 1.8 6.491853096 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Gluconic Acid sodium salt carbon source FEBA_gDNA_plate31 B11 IT023 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Gluconic Acid sodium salt 20 mM 0.02 1.77 6.46760555 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Gluconic Acid sodium salt carbon source FEBA_gDNA_plate31 B12 IT024 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Gluconic Acid sodium salt 20 mM 0.02 1.76 6.459431619 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Glucose-6-Phosphate sodium salt carbon source FEBA_gDNA_plate31 C1 IT025 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Glucose-6-Phosphate sodium salt 20 mM 0.02 2.99 7.224001674 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Glucose-6-Phosphate sodium salt carbon source FEBA_gDNA_plate31 C2 IT026 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Glucose-6-Phosphate sodium salt 20 mM 0.02 3 7.22881869 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Potassium acetate carbon source FEBA_gDNA_plate31 C3 IT027 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Potassium acetate 20 mM 0.02 0.55 4.781359714 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Potassium acetate carbon source FEBA_gDNA_plate31 C4 IT028 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Potassium acetate 20 mM 0.02 0.55 4.781359714 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Sodium Fumarate dibasic carbon source FEBA_gDNA_plate31 C5 IT029 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Sodium Fumarate dibasic 20 mM 0.02 0.57 4.832890014 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Sodium Fumarate dibasic carbon source FEBA_gDNA_plate31 C6 IT030 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Sodium Fumarate dibasic 20 mM 0.02 0.57 4.832890014 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Sodium D-Lactate carbon source FEBA_gDNA_plate31 C7 IT031 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Sodium D-Lactate 20 mM 0.02 0.88 5.459431619 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Sodium D-Lactate carbon source FEBA_gDNA_plate31 C8 IT032 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Sodium D-Lactate 20 mM 0.02 0.91 5.50779464 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Sodium L-Lactate carbon source FEBA_gDNA_plate31 C9 IT033 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Sodium L-Lactate 20 mM 0.02 0.8 5.321928095 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Sodium L-Lactate carbon source FEBA_gDNA_plate31 C10 IT034 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Sodium L-Lactate 20 mM 0.02 0.78 5.285402219 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Sodium D,L-Lactate carbon source FEBA_gDNA_plate31 C11 IT035 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Sodium D,L-Lactate 20 mM 0.02 0.68 5.09592442 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Sodium D,L-Lactate carbon source FEBA_gDNA_plate31 C12 IT036 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Sodium D,L-Lactate 20 mM 0.02 0.7 5.121015401 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Sodium pyruvate carbon source FEBA_gDNA_plate31 D1 IT037 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Sodium pyruvate 20 mM 0.02 0.54 4.754887502 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Sodium pyruvate carbon source FEBA_gDNA_plate31 D2 IT038 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Sodium pyruvate 20 mM 0.02 0.54 4.754887502 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Sodium succinate dibasic hexahydrate carbon source FEBA_gDNA_plate31 D3 IT039 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Sodium succinate dibasic hexahydrate 20 mM 0.02 0.81 5.339850003 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Sodium succinate dibasic hexahydrate carbon source FEBA_gDNA_plate31 D4 IT040 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Sodium succinate dibasic hexahydrate 20 mM 0.02 0.93 5.539158811 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Glycolic Acid carbon source FEBA_gDNA_plate31 D5 IT041 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Glycolic Acid 20 mM 0.02 0.38 4.263034406 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Glycolic Acid carbon source FEBA_gDNA_plate31 D6 IT042 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Glycolic Acid 20 mM 0.02 0.4 4.307428525 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Mannitol carbon source FEBA_gDNA_plate31 D7 IT043 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Mannitol 20 mM 0.02 1.64 6.357552005 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Mannitol carbon source FEBA_gDNA_plate31 D8 IT044 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Mannitol 20 mM 0.02 1.58 6.303780748 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Galacturonic Acid monohydrate carbon source FEBA_gDNA_plate31 D9 IT045 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Galacturonic Acid monohydrate 20 mM 0.02 1.66 6.371558863 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Galacturonic Acid monohydrate carbon source FEBA_gDNA_plate31 D10 IT046 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Galacturonic Acid monohydrate 20 mM 0.02 1.65 6.368069877 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Glycerol carbon source FEBA_gDNA_plate31 D11 IT047 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Glycerol 20 mM 0.02 0.92 5.529820947 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Glycerol carbon source FEBA_gDNA_plate31 D12 IT048 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm Glycerol 20 mM 0.02 0.91 5.510961919 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Time0 FEBA_gDNA_plate31 E1 IT049 LB flask Time0 37 Liquid Aerobic 200 rpm 0.02 2.23 6.8008999 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Time0 FEBA_gDNA_plate31 E2 IT050 LB flask Time0 37 Liquid Aerobic 200 rpm 0.02 2.23 6.8008999 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Sorbitol carbon source FEBA_gDNA_plate31 E3 IT051 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Sorbitol 20 mM 0.02 1.19 5.892391026 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Sorbitol carbon source FEBA_gDNA_plate31 E4 IT052 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Sorbitol 20 mM 0.02 1.14 5.837943242 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Malic acid disodium salt monohydrate carbon source FEBA_gDNA_plate31 E5 IT053 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm L-Malic acid disodium salt monohydrate 20 mM 0.02 0.56 4.817623258 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Malic acid disodium salt monohydrate carbon source FEBA_gDNA_plate31 E6 IT054 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm L-Malic acid disodium salt monohydrate 20 mM 0.02 0.6 4.906890596 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Trehalose dihydrate carbon source FEBA_gDNA_plate31 E7 IT055 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Trehalose dihydrate 20 mM 0.02 1.03 5.683696454 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Trehalose dihydrate carbon source FEBA_gDNA_plate31 E8 IT056 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Trehalose dihydrate 20 mM 0.02 1.01 5.655351829 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Serine carbon source FEBA_gDNA_plate31 E9 IT057 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Serine 20 mM 0.02 1.03 5.683696454 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Serine carbon source FEBA_gDNA_plate31 E10 IT058 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Serine 20 mM 0.02 1.1 5.781359714 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 casamino acids carbon source FEBA_gDNA_plate31 E11 IT059 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm casamino acids 2 mg/mL 0.02 0.76 5.255500733 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 casamino acids carbon source FEBA_gDNA_plate31 E12 IT060 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm casamino acids 2 mg/mL 0.02 0.77 5.263034406 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 N-Acetyl-D-Glucosamine carbon source FEBA_gDNA_plate31 F1 IT061 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm N-Acetyl-D-Glucosamine 20 mM 0.02 1.18 5.87774425 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 N-Acetyl-D-Glucosamine carbon source FEBA_gDNA_plate31 F2 IT062 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm N-Acetyl-D-Glucosamine 20 mM 0.02 1.2 5.902073579 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Glucosamine Hydrochloride carbon source FEBA_gDNA_plate31 F3 IT063 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Glucosamine Hydrochloride 20 mM 0.02 1.34 6.061776198 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Glucosamine Hydrochloride carbon source FEBA_gDNA_plate31 F4 IT064 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Glucosamine Hydrochloride 20 mM 0.02 1.34 6.06608919 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Mannose carbon source FEBA_gDNA_plate31 F5 IT065 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Mannose 20 mM 0.02 0.85 5.412781525 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Mannose carbon source FEBA_gDNA_plate31 F6 IT066 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Mannose 20 mM 0.02 0.88 5.452858965 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Galacturonic Acid monohydrate carbon source MOPS FEBA_gDNA_plate31 F7 IT067 MOPS Rich Defined media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Galacturonic Acid monohydrate 20 mM 0.02 1.13 5.820178962 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Galacturonic Acid monohydrate carbon source MOPS FEBA_gDNA_plate31 F8 IT068 MOPS Rich Defined media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Galacturonic Acid monohydrate 20 mM 0.02 1.4 6.129283017 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Glucose carbon source MOPS FEBA_gDNA_plate31 F9 IT069 MOPS Rich Defined media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Glucose 22 mM 0.02 0.734 5.197708158 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Glucose carbon source MOPS FEBA_gDNA_plate31 F10 IT070 MOPS Rich Defined media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Glucose 22 mM 0.02 0.83 5.375039431 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Arginine nitrogen source FEBA_gDNA_plate31 F11 IT071 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm L-Arginine 10 mM 0.02 0.67 5.06608919 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Arginine nitrogen source FEBA_gDNA_plate31 F12 IT072 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm L-Arginine 10 mM 0.02 0.65 5.022367813 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Aspartic Acid nitrogen source FEBA_gDNA_plate31 G1 IT073 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm L-Aspartic Acid 6 mM 0.02 0.99 5.626439137 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Aspartic Acid nitrogen source FEBA_gDNA_plate31 G2 IT074 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm L-Aspartic Acid 6 mM 0.02 1.01 5.658211483 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Serine nitrogen source FEBA_gDNA_plate31 G3 IT075 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm L-Serine 20 mM 0.02 0.92 5.529820947 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Serine nitrogen source FEBA_gDNA_plate31 G4 IT076 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm L-Serine 20 mM 0.02 0.92 5.529820947 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Asparagine nitrogen source FEBA_gDNA_plate31 G5 IT077 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm L-Asparagine 20 mM 0.02 1.21 5.916476644 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Asparagine nitrogen source FEBA_gDNA_plate31 G6 IT078 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm L-Asparagine 20 mM 0.02 1.23 5.942514505 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Glutamine nitrogen source FEBA_gDNA_plate31 G7 IT079 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm L-Glutamine 20 mM 0.02 1.66 6.371558863 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Glutamine nitrogen source FEBA_gDNA_plate31 G8 IT080 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm L-Glutamine 20 mM 0.02 1.7 6.409390936 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Glycine nitrogen source FEBA_gDNA_plate31 G9 IT081 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm Glycine 20 mM 0.02 1.1 5.776103988 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Glycine nitrogen source FEBA_gDNA_plate31 G10 IT082 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm Glycine 20 mM 0.02 1.1 5.776103988 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Alanine nitrogen source FEBA_gDNA_plate31 G11 IT083 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm L-Alanine 20 mM 0.02 1.04 5.700439718 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 L-Alanine nitrogen source FEBA_gDNA_plate31 G12 IT084 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm L-Alanine 20 mM 0.02 1 5.64385619 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Serine nitrogen source FEBA_gDNA_plate31 H1 IT085 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm D-Serine 20 mM 0.02 0.85 5.412781525 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Serine nitrogen source FEBA_gDNA_plate31 H2 IT086 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm D-Serine 20 mM 0.02 0.87 5.442943496 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Alanine nitrogen source FEBA_gDNA_plate31 H3 IT087 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm D-Alanine 20 mM 0.02 1.24 5.95419631 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 D-Alanine nitrogen source FEBA_gDNA_plate31 H4 IT088 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm D-Alanine 20 mM 0.02 1.21 5.918863237 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Gly-DL-Asp nitrogen source FEBA_gDNA_plate31 H5 IT089 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm Gly-DL-Asp 20 mM 0.02 2.52 6.977279923 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Gly-DL-Asp nitrogen source FEBA_gDNA_plate31 H6 IT090 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm Gly-DL-Asp 20 mM 0.02 2.33 6.864186145 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Gly-Glu nitrogen source FEBA_gDNA_plate31 H7 IT091 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm Gly-Glu 20 mM 0.02 0.51 4.672425342 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Gly-Glu nitrogen source FEBA_gDNA_plate31 H8 IT092 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm Gly-Glu 20 mM 0.02 0.52 4.700439718 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 casamino acids nitrogen source FEBA_gDNA_plate31 H9 IT093 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm casamino acids 2 mg/mL 0.02 2.18 6.768184325 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 casamino acids nitrogen source FEBA_gDNA_plate31 H10 IT094 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm casamino acids 2 mg/mL 0.02 2.16 6.754887502 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Putrescine Dihydrochloride nitrogen source FEBA_gDNA_plate31 H11 IT095 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm Putrescine Dihydrochloride 20 mM 0.02 0.6 4.916476644 Keio_ML9_set1 6/19/2014 Kelly Keio_ML9 Putrescine Dihydrochloride nitrogen source FEBA_gDNA_plate31 H12 IT096 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm Putrescine Dihydrochloride 20 mM 0.02 0.63 4.977279923 Keio_ML9_set2 6/19/2014 Kelly Keio_ML9 Time0 FEBA_gDNA_plate32 A1 IT001 LB flask Time0 37 Liquid Aerobic 200 rpm 0.02 2.23 6.8008999 Keio_ML9_set2 6/19/2014 Kelly Keio_ML9 Time0 FEBA_gDNA_plate32 A2 IT002 LB flask Time0 37 Liquid Aerobic 200 rpm 0.02 2.23 6.8008999 Keio_ML9_set2 6/19/2014 Kelly Keio_ML9 N-Acetyl-D-Glucosamine nitrogen source FEBA_gDNA_plate32 A3 IT003 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm N-Acetyl-D-Glucosamine 20 mM 0.02 1.3 6.022367813 Keio_ML9_set2 6/19/2014 Kelly Keio_ML9 N-Acetyl-D-Glucosamine nitrogen source FEBA_gDNA_plate32 A4 IT004 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm N-Acetyl-D-Glucosamine 20 mM 0.02 1.4 6.129283017 Keio_ML9_set2 6/19/2014 Kelly Keio_ML9 Adenosine nitrogen source FEBA_gDNA_plate32 A5 IT005 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm Adenosine 2 mM 0.02 0.66 5.044394119 Keio_ML9_set2 6/19/2014 Kelly Keio_ML9 Adenosine nitrogen source FEBA_gDNA_plate32 A6 IT006 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm Adenosine 2 mM 0.02 0.69 5.108524457 Keio_ML9_set2 6/19/2014 Kelly Keio_ML9 Cytidine nitrogen source FEBA_gDNA_plate32 A7 IT007 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm Cytidine 20 mM 0.02 1.32 6.048759312 Keio_ML9_set2 6/19/2014 Kelly Keio_ML9 Cytidine nitrogen source FEBA_gDNA_plate32 A8 IT008 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm Cytidine 20 mM 0.02 1.33 6.055282436 Keio_ML9_set2 6/19/2014 Kelly Keio_ML9 Ammonium chloride nitrogen source FEBA_gDNA_plate32 A9 IT009 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm Ammonium chloride 20 mM 0.02 1.22 5.935459748 Keio_ML9_set2 6/19/2014 Kelly Keio_ML9 Ammonium chloride nitrogen source FEBA_gDNA_plate32 A10 IT010 M9 minimal media_noNitrogen tube nitrogen source 37 Liquid Aerobic 200 rpm Ammonium chloride 20 mM 0.02 1.25 5.965784285 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 Time0 FEBA_gDNA_plate32 A11 IT011 LB flask Time0 37 Liquid Aerobic 200 rpm 0.02 1.5 6.22881869 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 Time0 FEBA_gDNA_plate32 A12 IT012 LB flask Time0 37 Liquid Aerobic 200 rpm 0.02 1.5 6.22881869 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Sodium nitrite 10 mM FEBA_gDNA_plate32 B1 IT013 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Sodium nitrite 10 mM 964 A1,A2 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Sodium nitrite 20 mM FEBA_gDNA_plate32 B2 IT014 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Sodium nitrite 20 mM 964 A3,A4 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Sodium nitrite 40 mM FEBA_gDNA_plate32 B3 IT015 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Sodium nitrite 40 mM 964 A5,A6 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Sodium nitrate 300 mM FEBA_gDNA_plate32 B4 IT016 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Sodium nitrate 300 mM 964 B1,B2 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Sisomicin sulfate salt 0.005 mg/ml FEBA_gDNA_plate32 B5 IT017 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Sisomicin sulfate salt 0.005 mg/ml 964 C1,C2 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Sodium Chlorite 0.4 mM FEBA_gDNA_plate32 B6 IT018 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Sodium Chlorite 0.4 mM 964 D1,D2 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Sodium Chlorite 1.25 mM FEBA_gDNA_plate32 B7 IT019 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Sodium Chlorite 1.25 mM 964 D3,D4 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Sodium perchlorate monohydrate 150 mM FEBA_gDNA_plate32 B8 IT020 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Sodium perchlorate monohydrate 150 mM 964 E1,E2 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Polymyxin B sulfate 0.0005 mg/ml FEBA_gDNA_plate32 B9 IT021 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Polymyxin B sulfate 0.0005 mg/ml 964 E7,E8 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Polymyxin B sulfate 0.001 mg/ml FEBA_gDNA_plate32 B10 IT022 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Polymyxin B sulfate 0.001 mg/ml 964 F1,F2 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Sodium Chloride 750 mM FEBA_gDNA_plate32 B11 IT023 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Sodium Chloride 750 mM 964 F3,F4 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB FEBA_gDNA_plate32 B12 IT024 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital 964 F7,F8 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Vancomycin Hydrochloride Hydrate 0.0625 mg/ml FEBA_gDNA_plate32 C1 IT025 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Vancomycin Hydrochloride Hydrate 0.0625 mg/ml 965 A1,A2 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Cephalothin sodium salt 0.01 mg/ml FEBA_gDNA_plate32 C2 IT026 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Cephalothin sodium salt 0.01 mg/ml 965 A7,A8 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Cephalothin sodium salt 0.08 mg/ml FEBA_gDNA_plate32 C3 IT027 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Cephalothin sodium salt 0.08 mg/ml 965 B1,B2 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Cephalothin sodium salt 0.16 mg/ml FEBA_gDNA_plate32 C4 IT028 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Cephalothin sodium salt 0.16 mg/ml 965 B3,B4 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with MreB Perturbing Compound A22 0.04 mg/ml FEBA_gDNA_plate32 C5 IT029 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital MreB Perturbing Compound A22 0.04 mg/ml 965 B5,B6 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with MreB Perturbing Compound A22 0.08 mg/ml FEBA_gDNA_plate32 C6 IT030 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital MreB Perturbing Compound A22 0.08 mg/ml 965 B7,B8 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Lomefloxacin hydrochloride 0.0004 mM FEBA_gDNA_plate32 C7 IT031 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Lomefloxacin hydrochloride 0.0004 mM 965 C5,C6 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Neomycin trisulfate salt hydrate 0.004 mg/ml FEBA_gDNA_plate32 C8 IT032 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Neomycin trisulfate salt hydrate 0.004 mg/ml 965 D7,D8 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB with Doxycycline hyclate 0.001 mg/ml FEBA_gDNA_plate32 C9 IT033 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Doxycycline hyclate 0.001 mg/ml 965 E5,E6 Keio_ML9_set2 6/24/2014 Kelly Keio_ML9 LB FEBA_gDNA_plate32 C10 IT034 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital 965 F7,F8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 Time0 FEBA_gDNA_plate32 C11 IT035 LB flask Time0 37 Liquid Aerobic 200 rpm 0.02 1.2 5.906890596 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 Time0 FEBA_gDNA_plate32 C12 IT036 LB flask Time0 37 Liquid Aerobic 200 rpm 0.02 1.2 5.906890596 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Nickel (II) chloride hexahydrate 1 mM FEBA_gDNA_plate32 D1 IT037 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Nickel (II) chloride hexahydrate 1 mM 960 A3,A4 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Cobalt chloride hexahydrate 0.32 mM FEBA_gDNA_plate32 D2 IT038 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Cobalt chloride hexahydrate 0.32 mM 960 B1,B2 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with copper (II) chloride dihydrate 2 mM FEBA_gDNA_plate32 D3 IT039 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital copper (II) chloride dihydrate 2 mM 960 B7,B8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with sodium fluoride 100 mM FEBA_gDNA_plate32 D4 IT040 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital sodium fluoride 100 mM 960 C5,C6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Thallium(I) acetate 0.0625 mg/ml FEBA_gDNA_plate32 D5 IT041 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Thallium(I) acetate 0.0625 mg/ml 960 D7,D8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Cisplatin 0.025 mg/ml FEBA_gDNA_plate32 D6 IT042 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Cisplatin 0.025 mg/ml 960 E5,E6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Cisplatin 0.05 mg/ml FEBA_gDNA_plate32 D7 IT043 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Cisplatin 0.05 mg/ml 960 E7,E8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Cisplatin 0.1 mg/ml FEBA_gDNA_plate32 D8 IT044 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Cisplatin 0.1 mg/ml 960 F1,F2 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB FEBA_gDNA_plate32 D9 IT045 LB 48 well microplate; Tecan Infinite F200 lb 28 Liquid Aerobic orbital 960 F7,F8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Benzalkonium Chloride 0.004 mg/ml FEBA_gDNA_plate32 D10 IT046 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Benzalkonium Chloride 0.004 mg/ml 961 A7,A8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Aluminum chloride hydrate 5 mM FEBA_gDNA_plate32 D11 IT047 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Aluminum chloride hydrate 5 mM 961 B5,B6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with benzoic acid 0.58 mg/ml FEBA_gDNA_plate32 D12 IT048 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital benzoic acid 0.58 mg/ml 961 C7,C8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Bacitracin 0.5 mg/ml FEBA_gDNA_plate32 E1 IT049 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Bacitracin 0.5 mg/ml 961 D7,D8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Bacitracin 1 mg/ml FEBA_gDNA_plate32 E2 IT050 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Bacitracin 1 mg/ml 961 E1,E2 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Fusidic acid sodium salt 0.03 mg/ml FEBA_gDNA_plate32 E3 IT051 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Fusidic acid sodium salt 0.03 mg/ml 961 E5,E6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Fusidic acid sodium salt 0.1 mg/ml FEBA_gDNA_plate32 E4 IT052 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Fusidic acid sodium salt 0.1 mg/ml 961 E7,E8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Dimethyl Sulfoxide 7.5 vol% FEBA_gDNA_plate32 E5 IT053 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Dimethyl Sulfoxide 7.5 vol% 961 F3,F4 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Dimethyl Sulfoxide 10 vol% FEBA_gDNA_plate32 E6 IT054 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Dimethyl Sulfoxide 10 vol% 961 F5,F6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB FEBA_gDNA_plate32 E7 IT055 LB 48 well microplate; Tecan Infinite F200 lb 28 Liquid Aerobic orbital 961 F7,F8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Chloramphenicol 0.001 mg/ml FEBA_gDNA_plate32 E8 IT056 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Chloramphenicol 0.001 mg/ml 959 A7,A8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Chloramphenicol 0.0025 mg/ml FEBA_gDNA_plate32 E9 IT057 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Chloramphenicol 0.0025 mg/ml 959 B1,B2 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Chloramphenicol 0.005 mg/ml FEBA_gDNA_plate32 E10 IT058 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Chloramphenicol 0.005 mg/ml 959 B3,B4 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Tetracycline hydrochloride 0.0004 mg/ml FEBA_gDNA_plate32 E11 IT059 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Tetracycline hydrochloride 0.0004 mg/ml 959 B5,B6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Tetracycline hydrochloride 0.0008 mg/ml FEBA_gDNA_plate32 E12 IT060 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Tetracycline hydrochloride 0.0008 mg/ml 959 B7,B8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Tetracycline hydrochloride 0.0016 mg/ml FEBA_gDNA_plate32 F1 IT061 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Tetracycline hydrochloride 0.0016 mg/ml 959 C1,C2 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Spectinomycin dihydrochloride pentahydrate 0.00625 mg/ml FEBA_gDNA_plate32 F2 IT062 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Spectinomycin dihydrochloride pentahydrate 0.00625 mg/ml 959 C3,C4 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Spectinomycin dihydrochloride pentahydrate 0.0125 mg/ml FEBA_gDNA_plate32 F3 IT063 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Spectinomycin dihydrochloride pentahydrate 0.0125 mg/ml 959 C5,C6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Carbenicillin disodium salt 0.004 mg/ml FEBA_gDNA_plate32 F4 IT064 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Carbenicillin disodium salt 0.004 mg/ml 959 D1,D2 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Carbenicillin disodium salt 0.008 mg/ml FEBA_gDNA_plate32 F5 IT065 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Carbenicillin disodium salt 0.008 mg/ml 959 D3,D4 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Carbenicillin disodium salt 0.016 mg/ml FEBA_gDNA_plate32 F6 IT066 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Carbenicillin disodium salt 0.016 mg/ml 959 D5,D6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Gentamicin sulfate salt 0.004 mg/ml FEBA_gDNA_plate32 F7 IT067 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Gentamicin sulfate salt 0.004 mg/ml 959 E7,E8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Gentamicin sulfate salt 0.008 mg/ml FEBA_gDNA_plate32 F8 IT068 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Gentamicin sulfate salt 0.008 mg/ml 959 F1,F2 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB FEBA_gDNA_plate32 F9 IT069 LB 48 well microplate; Tecan Infinite F200 lb 28 Liquid Aerobic orbital 959 F7,F8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with methylglyoxal 0.008 vol% FEBA_gDNA_plate32 F10 IT070 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital methylglyoxal 0.008 vol% 962 A7,A8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with 2-Furfuraldehyde 0.125 vol% FEBA_gDNA_plate32 F11 IT071 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital 2-Furfuraldehyde 0.125 vol% 962 B7,B8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with 2-Furfuraldehyde 0.25 vol% FEBA_gDNA_plate32 F12 IT072 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital 2-Furfuraldehyde 0.25 vol% 962 C1,C2 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with 5-Hydroxymethylfurfural 10 mM FEBA_gDNA_plate32 G1 IT073 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital 5-Hydroxymethylfurfural 10 mM 962 C5,C6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with 5-Hydroxymethylfurfural 20 mM FEBA_gDNA_plate32 G2 IT074 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital 5-Hydroxymethylfurfural 20 mM 962 C7,C8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Vanillin 5 mM FEBA_gDNA_plate32 G3 IT075 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Vanillin 5 mM 962 D1,D2 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with syringaldehyde 5 mM FEBA_gDNA_plate32 G4 IT076 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital syringaldehyde 5 mM 962 E5,E6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with syringaldehyde 10 mM FEBA_gDNA_plate32 G5 IT077 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital syringaldehyde 10 mM 962 E7,E8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Dimethyl Sulfoxide 10 vol% FEBA_gDNA_plate32 G6 IT078 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Dimethyl Sulfoxide 10 vol% 962 F5,F6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB FEBA_gDNA_plate32 G7 IT079 LB 48 well microplate; Tecan Infinite F200 lb 28 Liquid Aerobic orbital 962 F7,F8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Cholin acetate 75 mM FEBA_gDNA_plate32 G8 IT080 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Cholin acetate 75 mM 963 A7,A8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Cholin acetate 150 mM FEBA_gDNA_plate32 G9 IT081 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Cholin acetate 150 mM 963 B1,B2 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Sodium acetate 70 mM FEBA_gDNA_plate32 G10 IT082 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Sodium acetate 70 mM 963 B5,B6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with Sodium acetate 140 mM FEBA_gDNA_plate32 G11 IT083 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital Sodium acetate 140 mM 963 B7,B8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with 1-ethyl-3-methylimidazolium lysine 10 mM FEBA_gDNA_plate32 G12 IT084 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital 1-ethyl-3-methylimidazolium lysine 10 mM 963 C5,C6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with 1-ethyl-3-methylimidazolium acetate 30 mM FEBA_gDNA_plate32 H1 IT085 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital 1-ethyl-3-methylimidazolium acetate 30 mM 963 D3,D4 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with 1-ethyl-3-methylimidazolium acetate 60 mM FEBA_gDNA_plate32 H2 IT086 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital 1-ethyl-3-methylimidazolium acetate 60 mM 963 D5,D6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with 1-ethyl-3-methylimidazolium chloride 40 mM FEBA_gDNA_plate32 H3 IT087 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital 1-ethyl-3-methylimidazolium chloride 40 mM 963 D7,D8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with 1-ethyl-3-methylimidazolium chloride 80 mM FEBA_gDNA_plate32 H4 IT088 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital 1-ethyl-3-methylimidazolium chloride 80 mM 963 E1,E2 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB with L-Lysine 200 mM FEBA_gDNA_plate32 H5 IT089 LB 48 well microplate; Tecan Infinite F200 stress 28 Liquid Aerobic orbital L-Lysine 200 mM 963 F5,F6 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 LB FEBA_gDNA_plate32 H6 IT090 LB 48 well microplate; Tecan Infinite F200 lb 28 Liquid Aerobic orbital 963 F7,F8 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 Time0 FEBA_gDNA_plate32 H7 IT091 LB flask Time0 37 Liquid Aerobic 200 rpm 0.02 1.2 5.906890596 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 Time0 FEBA_gDNA_plate32 H8 IT092 LB flask Time0 37 Liquid Aerobic 200 rpm 0.02 1.2 5.906890596 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 D-Galacturonic Acid monohydrate carbon source MOPS FEBA_gDNA_plate32 H9 IT093 MOPS minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Galacturonic Acid monohydrate 20 mM 0.02 2.71 7.082149041 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 D-Glucose carbon source MOPS FEBA_gDNA_plate32 H10 IT094 MOPS minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Glucose 22 mM 0.02 2.4 6.906890596 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 D-Galacturonic Acid monohydrate carbon source M9 FEBA_gDNA_plate32 H11 IT095 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Galacturonic Acid monohydrate 20 mM 0.02 2.11 6.721099189 Keio_ML9_set2 6/30/2014 Kelly Keio_ML9 D-Glucose carbon source M9 FEBA_gDNA_plate32 H12 IT096 M9 minimal media_noCarbon tube carbon source 37 Liquid Aerobic 200 rpm D-Glucose 22 mM 0.02 1.4 6.129283017 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB with Nalidixic acid sodium salt 0.006 mg/ml FEBA_gDNA_plate64 E1 IT049 LB 48 well microplate; Tecan Infinite F200 stress 25 Liquid Aerobic orbital Nalidixic acid sodium salt 0.006 mg/ml 1134 A5,A6 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB with Benzethonium chloride 0.0125 mM FEBA_gDNA_plate64 E2 IT050 LB 48 well microplate; Tecan Infinite F200 stress 25 Liquid Aerobic orbital Benzethonium chloride 0.0125 mM 1134 A7,A8 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB with Phosphomycin disodium salt 0.0005 mg/ml FEBA_gDNA_plate64 E3 IT051 LB 48 well microplate; Tecan Infinite F200 stress 25 Liquid Aerobic orbital Phosphomycin disodium salt 0.0005 mg/ml 1134 B5,B6 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB with Phosphomycin disodium salt 0.001 mg/ml FEBA_gDNA_plate64 E4 IT052 LB 48 well microplate; Tecan Infinite F200 stress 25 Liquid Aerobic orbital Phosphomycin disodium salt 0.001 mg/ml 1134 B7,B8 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB with Phosphomycin disodium salt 0.002 mg/ml FEBA_gDNA_plate64 E5 IT053 LB 48 well microplate; Tecan Infinite F200 stress 25 Liquid Aerobic orbital Phosphomycin disodium salt 0.002 mg/ml 1134 C1,C2 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB with D-Cycloserine 0.1 mg/ml FEBA_gDNA_plate64 E6 IT054 LB 48 well microplate; Tecan Infinite F200 stress 25 Liquid Aerobic orbital D-Cycloserine 0.1 mg/ml 1134 C7,C8 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB FEBA_gDNA_plate64 E7 IT055 LB 48 well microplate; Tecan Infinite F200 lb 25 Liquid Aerobic orbital 1134 F7,F8 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB pH5 FEBA_gDNA_plate64 E8 IT056 LB 48 well microplate; Tecan Infinite F200 pH 25 5 Liquid Aerobic orbital 1135 A2,B2 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB pH5 FEBA_gDNA_plate64 E9 IT057 LB 48 well microplate; Tecan Infinite F200 pH 25 5 Liquid Aerobic orbital 1135 C2,D2 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB pH5 FEBA_gDNA_plate64 E10 IT058 LB 48 well microplate; Tecan Infinite F200 pH 25 5 Liquid Aerobic orbital 1135 E2,F2 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB pH6 FEBA_gDNA_plate64 E11 IT059 LB 48 well microplate; Tecan Infinite F200 pH 25 6 Liquid Aerobic orbital 1135 A3,B3 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB pH6 FEBA_gDNA_plate64 E12 IT060 LB 48 well microplate; Tecan Infinite F200 pH 25 6 Liquid Aerobic orbital 1135 C3,D3 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB pH6 FEBA_gDNA_plate64 F1 IT061 LB 48 well microplate; Tecan Infinite F200 pH 25 6 Liquid Aerobic orbital 1135 E3,F3 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB pH7 FEBA_gDNA_plate64 F2 IT062 LB 48 well microplate; Tecan Infinite F200 pH 25 7 Liquid Aerobic orbital 1135 A4,B4 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB pH7 FEBA_gDNA_plate64 F3 IT063 LB 48 well microplate; Tecan Infinite F200 pH 25 7 Liquid Aerobic orbital 1135 C4,D4 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB pH7 FEBA_gDNA_plate64 F4 IT064 LB 48 well microplate; Tecan Infinite F200 pH 25 7 Liquid Aerobic orbital 1135 E4,F4 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB pH8 FEBA_gDNA_plate64 F5 IT065 LB 48 well microplate; Tecan Infinite F200 pH 25 8 Liquid Aerobic orbital 1135 A5,B5 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB pH8 FEBA_gDNA_plate64 F6 IT066 LB 48 well microplate; Tecan Infinite F200 pH 25 8 Liquid Aerobic orbital 1135 C5,D5 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 LB pH8 FEBA_gDNA_plate64 F7 IT067 LB 48 well microplate; Tecan Infinite F200 pH 25 8 Liquid Aerobic orbital 1135 E5,F5 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 outer cut, LB soft agar motility assay FEBA_gDNA_plate64 F8 IT068 LB soft agar motility 30 Liquid Aerobic 0 rpm Keio_ML9_set6 2/2/2015 Mark Keio_ML9 outer cut, LB soft agar motility assay FEBA_gDNA_plate64 F9 IT069 LB soft agar motility 30 Liquid Aerobic 0 rpm Keio_ML9_set6 2/2/2015 Mark Keio_ML9 outer cut, LB soft agar motility assay FEBA_gDNA_plate64 F10 IT070 LB soft agar motility 30 Liquid Aerobic 0 rpm Keio_ML9_set6 2/2/2015 Mark Keio_ML9 outer cut, LB soft agar motility assay FEBA_gDNA_plate64 F11 IT071 LB soft agar motility 30 Liquid Aerobic 0 rpm Keio_ML9_set6 2/2/2015 Mark Keio_ML9 inner cut, LB soft agar motility assay FEBA_gDNA_plate64 F12 IT072 LB soft agar motility 30 Liquid Aerobic 0 rpm Keio_ML9_set6 2/2/2015 Mark Keio_ML9 inner cut, LB soft agar motility assay FEBA_gDNA_plate64 G1 IT073 LB soft agar motility 30 Liquid Aerobic 0 rpm Keio_ML9_set6 2/2/2015 Mark Keio_ML9 inner cut, LB soft agar motility assay FEBA_gDNA_plate64 G2 IT074 LB soft agar motility 30 Liquid Aerobic 0 rpm Keio_ML9_set6 2/2/2015 Mark Keio_ML9 inner cut, LB soft agar motility assay FEBA_gDNA_plate64 G3 IT075 LB soft agar motility 30 Liquid Aerobic 0 rpm Keio_ML9_set6 2/2/2015 Mark Keio_ML9 Time0 FEBA_gDNA_plate64 G4 IT076 LB flask Time0 30 Liquid Aerobic 200 rpm 0.02 1.1 5.781359714 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 Time0 FEBA_gDNA_plate64 G5 IT077 LB flask Time0 30 Liquid Aerobic 200 rpm 0.02 1.1 5.781359714 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 Time0 FEBA_gDNA_plate64 G6 IT078 LB flask Time0 30 Liquid Aerobic 200 rpm 0.02 1.1 5.781359714 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 Time0 FEBA_gDNA_plate64 G7 IT079 LB flask Time0 30 Liquid Aerobic 200 rpm 0.02 1.1 5.781359714 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 Time0 FEBA_gDNA_plate64 G8 IT080 LB flask Time0 30 Liquid Aerobic 200 rpm 0.02 1.1 5.781359714 Keio_ML9_set6 2/2/2015 Mark Keio_ML9 Time0 FEBA_gDNA_plate64 G9 IT081 LB flask Time0 30 Liquid Aerobic 200 rpm 0.02 1.1 5.781359714